serial dilution
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Dilution problem.
1. Prepare a serial 5 fold dilution to 1: 3125
Microbiology: How do you calculate original concentration from serial dilution plating?
You are given an unknown concentration of cells in a tube and asked to do a serial dilution to obtain a final dilution of 1:10,000. You have 2 additional empty tubes and are asked to make the final volume for each dilution 100 microliters. You will be using phosphate-buffered saline (PBS) to dilute the cells. Explain, in words or diagram, how would you perform this serial dilution to obtain a final dilution of 1:10,000. Make sure you indicate the volume of cells added to each tube, and the volume of PBS in each tube in your answer.
