Question

LAB MODULE: BASIC MICROSCOPY – STUDY HELP

The information in this document is helpful in preparing for the Basic Microscopy Pre-Lab Quiz on Canvas and the Basic Microscopy quiz on Late Nite Labs.

Instructions:

• Go to your Late Nite Labs course site and click on the Basic Microscopy link. The lab manual content will appear on the right side of the screen.

• PRINT and READ all of the information found in the Basic Microscopy lab manual.

• Use the information to answer the following questions.

Questions

• Fill in the remainder of the chart.

Objective Lens	Objective Magnification	Ocular Magnification	Total Magnification
Scanning	4X	10X	40X
Low Power	10X	10X
High Power	40X	10X
Oil Immersion	100X	10X

• Do all organisms consist of more than one cell?

• What is the structural and functional unit of living things? In other words, what is considered the basic building block of LIFE?

• What three structures are found in the cells of ALL organisms, both prokaryotic and eukaryotic? You may have to look at the pictures of the cells in the Basic Microscopy lab manual content to find all three structures. Note: plasma membrane = cell membrane

• List the major differences in prokaryotic cells and eukaryotic cells? Include the location of genetic material in a eukaryotic versus prokaryotic cell.

• List the organelles found in a eukaryotic cell that are not found in a prokaryotic cell. You may have to look at the pictures of the cells in the Basic Microscopy lab manual content to find all of these.

• In eukaryotic cells, genetic information is found in the form of DNA. Is this true for prokaryotic cells?

• Are flagella and cell walls unique to eukaryotes? Why or why not?

• The __________is the part of a cell that allows the movement of substances into and out of the cell and protects the cell from its environment.

• The__________is a substance that contains all of the dissolved and suspended ions and other molecules needed to keep the cell alive.

• A__________is an organelle that serves as the site of photosynthesis in a eukaryotic cell.

• Some organisms have cells that contain a__________that gives the cell a more structured shape and strengthens the cell by osmotic pressure.

• Describe dark field microscopy.

• Describe phase contrast microscopy.

• Why do scientists use dark field microscopy and phase contrast microscopy when studying prokaryotic cells?

• How does phase contrast microscopy help scientists visualize difficult specimens?

• Fill in the remainder of the chart. The organisms studied during this lab are: Amoeba, Spirogyra, cardiac muscle, and bacteria.

Structure	Function	Organisms Studied
Plasma Membrane		Amoeba, Spirogyra, cardiac muscle, and bacteria
Cytoplasm
Nucleus	Contains the genetic material of a eukaryotic cell
Chloroplast		Spirogyra
Cell wall