Cloning has been seen in the news and as a major topic of debate.

Briefly describe the process of cloning.

What might be the benefits of cloning?

What are potential dangers or threats associated with the widespread use of cloning?

Be prepared to discuss the ethics of cloning in its various proposed forms and uses.

Please answer each question.

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Reid WolffLv2

15 Apr 2018

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Related questions

Cloning is a major topic of debate and is described in the textbook. Briefly describe the process of cloning in the lab incorporating facts from the textbook.

How is cloning involved in gene therapy?

Give a specific and detailed example of how Gene therapy may be used to solve problems associated with genetic disorders. Discuss the benefits and possible hazards of Gene therapy to human health.

What might be the benefits of cloning?

What are potential dangers or treats associated with the wide spread use of cloning?

Discuss the ethics of cloning in its various proposed forms and uses.

Submission Details:

Post your response to Discussion Area by the Due Date assigned. Respond to at least two posts by the end of the week.

sapphireduck935

Question: Think about these questions, and post your thoughts on the use of cloning. Specifically, think the about the use of human cloning and discuss the potential impact on society.

Background Discussion: The scientific research terms, cloning means making exact copies of genes, a cell, or an organism. Therefore, cloning has been around for a long time. Identical twins are clones of a single zygote. When a single bacterium reproduces asexually on a petri dish, a colony of cells results, and each member of the colony is a clone of the original cell. Through biotechnology, bacteria now produce clone copies of human genes.

Through recent advances in science, it is possible to produce a clone of a mammal- and perhaps even one day, a human. The parent need not contribute sperm or egg to the process. During the so-called reproductive cloning, a nucleus (which contains the genetic material of a person) from one adult cell is placed in an enucleated egg. That egg is then allowed to develop. The developing embryo is placed in the uterus of a surrogate mother, and when birth occurs, the clone is an exact genetic copy of the original parent (of course the clone is younger). The process of cloning whole animals, and certainly humans, has not been perfected. Until it is, therapeutic cloning is more likely to become widespread.

Suppose it were possible to put the nucleus from a cell of a burn victim into an enucleated egg that is forced to become skin cells in the laboratory. These cells could be used to provide grafts of new skin that would not be rejected by the recipient. Would this be a proper use of cloning in humans?

Now, suppose parents want to produce a child free of genetic diseases. Scientists produce an embryo though in vitro fertilization, and then they clone the embryo to produce several embryos. Genetic engineering is then used to remove the genetic defect, and the defect free embryos are implanted into the uterus of a surrogate where they are allowed to grow to term. Would this be proper use of cloning in humans?

What if later scientists were able to produce children with increased intelligence or athletic abilities using the same technique? Would this be an acceptable use of cloning in humans?

This experiment is about Affinity Purification of Green Fluorescent Protein (GFP)

please answer the below questions

1. What is a bacterial colony?

2. Why did you pick one green colony and one white colony from your agar plate(s)? Why do you think you picked one of each color? What could this prove?

3. How are these items helpful in this cloning experiment? a. ultraviolet (UV) light - b. incubator - c. shaking incubator

4. Explain how placing cloned cells in nutrient broth to multiply relates to your overall goal of purifying the fluorescent protein.

5. You have used a bacterium to propagate a gene that produces a green fluorescent protein. Identify the function of these items you need in this procedure. a. Centrifuge â b. Lysozyme â c. Freezer â

6. Can you explain why both liquid cultures fluoresce green?

7. Why did you discard the supernatant in this part of the protein purification procedure?

8. Can you explain why the bacterial cellsâ outer membrane ruptures when the cells are frozen. What happens to an unopened soft drink when it freezes?

9. What was the purpose of rupturing or lysing the bacteria?

10. Briefly describe hydrophobic interaction chromatography and identify its purpose in this lab.

This lab is about Crystal Growth of Hen Egg White Lysozyme so please answer the following question

1. What condition yielded the "best" crystals of lysozyme?

2. What changes did your group try to make, and what effect did they have on the crystals?

3. How many of your conditions yielded crystals? How many did the entire class get? Give the percentage (number of conditions that gave crystals/number of different conditions that were set up) for your attempts as well as for the entire class. What does this tell you about protein crystallization?

4. What methods can you think of to determine if the crystals are lysozyme and not salt (many salt crystals are colorless as well)? (Hint: think of protein properties and ways to assay and look at proteins).

5. Many protein crystals are formed at or near the pI of the protein being studied. Can you rationalize any reason why this observation might be made (in other words, why is the pI so good at helping form protein crystals).