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11 Jun 2018
Plaease help me to answer these question of gel electrophresis class.
Q1: What is electrophresis and why do you need to run a DNA ladder together with your sample ?
Q2 In the well where you ran pUC19 (plasmid sample with known concentration for positive contorol) , you will probably see more than one band. The purification with the silica column allows for the elimination of chromosomal contaminants, so what types of DNA molecules might be present in the different bands ( In order to ansewer this question, you need to find out how plasmids repilicate and what the properities of supercoiled plasmids are)
I can ansewer to Q1 by myself (I also asked for Q1, because I want to check my own ansewer) , but to Q2, I can't.
Plaease help me to answer these question of gel electrophresis class.
Q1: What is electrophresis and why do you need to run a DNA ladder together with your sample ?
Q2 In the well where you ran pUC19 (plasmid sample with known concentration for positive contorol) , you will probably see more than one band. The purification with the silica column allows for the elimination of chromosomal contaminants, so what types of DNA molecules might be present in the different bands ( In order to ansewer this question, you need to find out how plasmids repilicate and what the properities of supercoiled plasmids are)
I can ansewer to Q1 by myself (I also asked for Q1, because I want to check my own ansewer) , but to Q2, I can't.
Keith LeannonLv2
13 Jun 2018
