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In a short paragraph or two, contrast each of the following pairs of concepts:

a. complementation vs. strain

b. YPD media vs. SD media

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Reid Wolff
Reid WolffLv2
1 Jan 2019

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Related questions

5. All of your YPD patches will be replica printed onto a MATa lawn and onto a MATalpha lawn. Each of those replicas will be incubated for 48 hours and then printed onto SD. You will therefore have two SD plates, one originating from the MATa tester replica and one originating from the MATalpha tester replica.

a. Should any of the specific patches that arise on the SD plate originating from the MATa tester replica also arise on the SD plate originating from the MATalpha tester replica? (i think the answer is yes)

b. If you have a diploid patch on your YPD patch plate, will that colony result in growth on the SD plate originating from the MATa tester replica?

c. If you have a diploid patch on your YPD patch plate, will that colony result in growth on the SD plate originating from the MATalpha tester replica?

Compare and contrast the following terms and concepts, in the context of this course (4 points each, <100 words)

Likelihood vs. Bayesian analysis

Taxonomy vs. nomenclature

1. Explain briefly how Lederberg and Tatum established that the sexual process occurred in E. coli:

2. Answer the following questions for the S. cerevisiae practical: Only spontaneous mutants unable to convert α-aminoadipate to α-aminoadipate-semialdehyde will be able to grow on the α-aminoadipate media, where they use this compound as a nitrogen source.

（1） Where do these mutants come from?

（2） Do they form on the α-aminoadipate media, or are they already present on the YPD plate?

（3）Are there likely any other types of mutants present? Why?

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