When white blood cells are physically disrupted (organelles are released) in a blender containing distilled water and then added to an acidic buffer, the hydrolytic activity of the suspension is much greater than when organelles are released in a blender co ntaining an isotonic solution adn then added to the same acidic buffer. What does "hydrolytic activity" mean and why should the organelles released into the distilled water have higher hydrolytic activity under acid conditions than the organelles released into an isotonic solution?
It was discovered that a fraction of membrane proteins can be removed by immersing a membrane in an aqueous salt solution, whereas the remaining fraction of proteins can only be removed by immersing the membrane in a detergen solution or organic solvent which dissolves lipids. Which fraction contains the peripheral proteins and which fraction contains the integral proteins and why? What does this experiment say about the way peripheral and integral proteins are bound to membranes?
When white blood cells are physically disrupted (organelles are released) in a blender containing distilled water and then added to an acidic buffer, the hydrolytic activity of the suspension is much greater than when organelles are released in a blender co ntaining an isotonic solution adn then added to the same acidic buffer. What does "hydrolytic activity" mean and why should the organelles released into the distilled water have higher hydrolytic activity under acid conditions than the organelles released into an isotonic solution?
It was discovered that a fraction of membrane proteins can be removed by immersing a membrane in an aqueous salt solution, whereas the remaining fraction of proteins can only be removed by immersing the membrane in a detergen solution or organic solvent which dissolves lipids. Which fraction contains the peripheral proteins and which fraction contains the integral proteins and why? What does this experiment say about the way peripheral and integral proteins are bound to membranes?