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28 Sep 2018

All the DNA extraction protocols I have seen involve addingsalts to the extraction buffer. What is the purpose of the salts?What happens if they aren't included?

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I need help with these questions about Genetics Laboratory techniques involving DNA extraction please:

1.) We add RNase solution to the extraction cocktail (tissue and lysis buffer) in order to:

a. Degrade RNase to remove remaining RNase

b. Prevent degradation of DNA to increase yield of DNA

c. Remove any RNA from the extraction cocktail to ensure isolation of deoxynucleic acid molecules

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2.) After precipitation of DNA and centrifugation at maximum speed, a “supernatant” is produced. The “supernatant” is the portion that contains DNA but not the pellet.

a. True

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3.) After precipitation of DNA and centrifugation at maximum speed, the supernatant is discarded.

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4.) ____________ precipitation of DNA and centrifugation at maximum speed and washing the pellet using ethanol, _________________ is added to the pellet to elute the DNA in the solution.

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