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20 Oct 2018

I am trying to convert a set of Affymetrix ID's, like this one143053_at_3745, to Flybase Gene Numbers (FBgn) like this oneFBgn0000015. I have downloaded the Flybase file required to do so(as described here) but I have noticed that most of the FBgn's havemore than one associated Affy id.

My question is, how do I know which to assign to my data? Fromthe list of affy-ids that I have, how do I label each one with theappropriate FBgn? I am using data from the Ayroles et al 2009(DGRP) which has a column of AffyID which look similar to the onesin the Flybase file but shorter (1638273_at). Perhaps I don't graspwhy there would be more than one.

Some have one affy id which is repeated across 15 columns in thefile, whereas some seem to have several affy id's associated withthem.

Some questions this problem raises:

Why does a singular FBgn identifier have more than oneassociated affy identifier?

Why do some datasets, such as the dataset I have, have shorterversions of the affy identifier?

Where can I find an up to date and appropriate list matchingthese Affy id's the FBgn identifiers correctly?

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Beverley Smith
Beverley SmithLv2
23 Oct 2018

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