Question

I am conducting a biochemistry-related experiment and I havebeen unable to understand a step which is commonly performed. Myaim in this step is to apply a PEG (Polyethylene glycol) silanelayer.

After immersing indium tin oxide (ITO) slides in aPEG-concentration, the slides are incubated for 18 hours at atemperature of 60 degrees Celsius.

Can you tell me why the incubation is performed? And why for 18hours and at 60 degrees Celsius?

Thanks a lot in advance!!