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18 Jan 2018
In Proteomics Practicals
a) Why is it necessary to determine the amount and concentration of protein in a sample extract? (short answer)
b) If you use a number of different assays to determine the amount of protein in a sample, would you expect the value to be exactly the same? Explain your answer.
c) If you use a number of different assays to determine the amount of protein in a sample, would you expect the relative values between samples to be the same? In other words, if two samples had a relative total protein amount of 1:2 (twice as much in sample 2) in one assay, would the relative total amount determined by another assay be 1:2?
In Proteomics Practicals
a) Why is it necessary to determine the amount and concentration of protein in a sample extract? (short answer)
b) If you use a number of different assays to determine the amount of protein in a sample, would you expect the value to be exactly the same? Explain your answer.
c) If you use a number of different assays to determine the amount of protein in a sample, would you expect the relative values between samples to be the same? In other words, if two samples had a relative total protein amount of 1:2 (twice as much in sample 2) in one assay, would the relative total amount determined by another assay be 1:2?
Bunny GreenfelderLv2
19 Jan 2018
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