In quantitative (qPCR), or âreal-timeâ PCR, define the meaning of the Ct value?
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Is it possible to use a biologically active TelemoreaseElongation Reverse Transcriptase (TERT) in the place of the ReverseTranscriptase (RT) for quantitative Reverse Transcriptase pcr(qRT-pcr)? Yes I do know that it would seemingly have noadvantages. What kind of reverse transcriptase is used in qRT-pcris there a standard like taq dna pol is used for qpcr?
1. How can ELISA be used for âpost-infectionâ diagnosis of a disease, that is, the antigen is already undetectable in the body?
2. Differentiate the results that can be obtained from an experiment using traditional PCR and qPCR in GMO detection. What is the advantage of the latter? Why is gel electrophoresis sometimes still done after amplification with qPCR?