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30 Oct 2018
Cytochrome b5 is red, which makes it handy for us as we can see the His-tagged version bind to the column â it forms a dark red band at the top of the NiNTA column. Suppose you applied your E. coli supernatant containing your cytochrome b5 to the column, and to your horror no such band forms: it just passes through the column and ends up in the unbound fraction. Now you are in trouble-shooting mode: what are two plausible explanations for this unexpected behavior?
Cytochrome b5 is red, which makes it handy for us as we can see the His-tagged version bind to the column â it forms a dark red band at the top of the NiNTA column. Suppose you applied your E. coli supernatant containing your cytochrome b5 to the column, and to your horror no such band forms: it just passes through the column and ends up in the unbound fraction. Now you are in trouble-shooting mode: what are two plausible explanations for this unexpected behavior?
Patrina SchowalterLv2
31 Oct 2018
