The trp-cage protein is a tiny protein with a 20 amino acid sequence. The sequence (starting from the N terminus) is NLYIQWLKDGGPSSGRPPPS
a. Using the sequence, write the mRNA code corresponding to trp-cage. Write it in the 5â-3â direction.
b. Write the DNA sequence corresponding to the trp-cage. Specify the 5â-3â direction.
c. Explain the steps from transcription to translation to generate trp-cage from the original DNA sequence. Be specific about direction (5â-3â) in which mRNA comes off of the DNA. Show where a small piece of the mRNA is complementary to the original DNA in the correct direction. Where in the cell do these steps take place? Basically, demonstrate that you fully understand exactly how transcription and translation take place. You do NOT need to name every enzyme. Just the major ones.
d. There are no introns in DNA sequence you derived from the known protein structure. Why not?
The trp-cage protein is a tiny protein with a 20 amino acid sequence. The sequence (starting from the N terminus) is NLYIQWLKDGGPSSGRPPPS
a. Using the sequence, write the mRNA code corresponding to trp-cage. Write it in the 5â-3â direction.
b. Write the DNA sequence corresponding to the trp-cage. Specify the 5â-3â direction.
c. Explain the steps from transcription to translation to generate trp-cage from the original DNA sequence. Be specific about direction (5â-3â) in which mRNA comes off of the DNA. Show where a small piece of the mRNA is complementary to the original DNA in the correct direction. Where in the cell do these steps take place? Basically, demonstrate that you fully understand exactly how transcription and translation take place. You do NOT need to name every enzyme. Just the major ones.
d. There are no introns in DNA sequence you derived from the known protein structure. Why not?
Related textbook solutions
Related questions
DNA Structure and Function LabReport
- DNA Structure
- Which two scientists are credited with discovering DNA?
- Name the nitrogen bases that are purines.
- Which nitrogen base pairs with thymine?
- List the three components of a nucleotide.
- DNA Replication
- What is the purpose of DNA replication?
- How many times does replication occur in the life of acell?
- In the Lab, Exercise 2, the original strand on the left had thebases shown below. Input the new bases that correctly pair with theoriginal strand.
Original | New |
C | |
A | |
G | |
T |
- RNA Structure
- Describe the structure and function of RNA.
- Refer to Exercise 3 and record the bases of the RNA strandproduced from the replicated DNA strand.
DNA | RNA |
C | |
A | |
G | |
T |
- Record the differences between DNA and RNA in the tablebelow.
DNA | RNA | |
Sugars | ||
Bases | ||
Strands |
- RNA Synthesis
- The process of assembling RNA is called _________.
- How is replication different from transcription?
- Refer to Exercise 4. Write the letters for the base sequence ofmRNA in the spaces below DNA. Note that the order is reversed;start with the 3â end of the DNA strand and the 5â end of the mRNAstrand. Transcription is DNA to mRNA. Note RNA contains Uracilinstead of Thymine; There is no thymine in RNA.
DNA | 3â | C | G | T | C | G | T | C | C | A | A | T | T | 5â |
mRNA | 5â | 3â |
- Protein Synthesis
- What type of RNA provides amino acids to build polypeptidechains?
- If a mRNA strand has the bases 5â CUC 3â, what amino acid willbe translated? Refer to the printable chart in Exercise 5.
- Where in the cell does translation occur?
- Genes
- What could be the problem if there is a change in the basesequence of a gene as it is passed down to the offspring?
- Give an example of a disorder that results from changes in theamino acid sequence.
- What causes sickle cell anemia?
- Gene Cloning
- What is the function of a plasmid?
- Print the document from Lab, Exercise 6. Complete the activityalong with the video demonstration. Sign, date, and take an imageof your final product and include with this lab report.
