1
answer
0
watching
67
views
12 Feb 2019

Design the primers that you will use for the PCR reaction to amplify the rac gene. Include any additional sequences (for example restriction enzyme sites). (b) (5 points) – Suppose that when you designed your primers, you made a mistake and instead of the correct base (a G) on the 3' end of one of the primers you placed a C nucleotide; explain how this would affect the results of the PCR (if no affect, state that). (c) The PCR product does not contain phosphates on their 5’ends. How can it be ligated into the AT-vector?

For unlimited access to Homework Help, a Homework+ subscription is required.

Trinidad Tremblay
Trinidad TremblayLv2
13 Feb 2019

Unlock all answers

Get 1 free homework help answer.
Already have an account? Log in

Related textbook solutions

Related questions

Weekly leaderboard

Start filling in the gaps now
Log in