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12 Feb 2019
Design the primers that you will use for the PCR reaction to amplify the rac gene. Include any additional sequences (for example restriction enzyme sites). (b) (5 points) â Suppose that when you designed your primers, you made a mistake and instead of the correct base (a G) on the 3' end of one of the primers you placed a C nucleotide; explain how this would affect the results of the PCR (if no affect, state that). (c) The PCR product does not contain phosphates on their 5âends. How can it be ligated into the AT-vector?
Design the primers that you will use for the PCR reaction to amplify the rac gene. Include any additional sequences (for example restriction enzyme sites). (b) (5 points) â Suppose that when you designed your primers, you made a mistake and instead of the correct base (a G) on the 3' end of one of the primers you placed a C nucleotide; explain how this would affect the results of the PCR (if no affect, state that). (c) The PCR product does not contain phosphates on their 5âends. How can it be ligated into the AT-vector?
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Trinidad TremblayLv2
13 Feb 2019