1.Why is it that golden gate reactions are able to have both restriction digest reactions and ligation reactions take place in the same tube? Is there a possibility that vectors that already have the insert will be undone?
1.Why is it that golden gate reactions are able to have both restriction digest reactions and ligation reactions take place in the same tube? Is there a possibility that vectors that already have the insert will be undone?
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Conservative transposonsare
excised from donor chromosome and insert into their recipientDNA molecule, leaving a double strand break in the donorchromosome | |||||||||
Duplicated from donor chromosome and inserted into theirrecipient DNA molecule | |||||||||
Duplicated from donor chromosome and inserted into theirrecipient DNA molecule, leaving a double strand break in the donorchromosome | |||||||||
| excised from donor chromosome and insert into their recipientDNA molecule, causing re-ligation of the nick in the donorchromosome. Restriction enzymes that generate single strandoverhangs are preferred for ligations because
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