1. Why do organisms carrying out nitrogen fixation require an environment with reduced levels of oxygen?
2. Provide two examples of how organisms fixing nitrogen avoid the "oxygen problem" that you discussed in your answer to #1.
1. Why do organisms carrying out nitrogen fixation require an environment with reduced levels of oxygen?
2. Provide two examples of how organisms fixing nitrogen avoid the "oxygen problem" that you discussed in your answer to #1.
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1. Complete the following chart:
Organism | Metabolizes oxygen | Growth in presence of oxygen | Growth in absence of oxygen | detoxifies byproduct of oxygen |
Aerobe | ||||
Facultative anaerobe | ||||
Strict anaerobe | ||||
Aerotolerant | ||||
Microaerophile |
2. Compare the results with growth on the aerobic control plate and describe the oxygen requirements of your organisms.
3. If an organism is a strict anaerobe, it cannot survive in the presence of oxygen even for a short time. How might you culture these organisms?
4. An aerobe, facultative anaerobe, strict anaerobe, and an aerotolerant anaerobe are inoculated on a medium in a sealed container with oxygen present. Explain what will happen.