1) ESSAY[20 PTS]- A living cell is constantly "fighting" entropy. What does this mean in terms of a cell and how does the cell carry this out? Be sure to write legibly and in complete sentences in 4 to 5 paragraphs

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Casey DurganLv2

2 Nov 2018

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1. What are the structural and functional units of a living organism? 1. What are the structural and functional units of a living organism? 2. What is the role of the cell membrane? 3. What is the cell membrane made of? 4. What does phospholipid bi-layer mean? 5. What are some functions of membrane proteins? 6. What are the two major groups of cell membrane proteins? How are they different? 7. Describe diffusion. 8. What does selectively permeable mean? 9. Does diffusion require energy from the cell? 10. What might restrict the movement of a molecule across a cell membrane? 11. In the video what are channel proteins are compared to? 12. What does gated channel mean? 13. Explain osmosis. 14. Explain isotonic. 15. Explain hypotonic. 16. Explain lysis of a cell. 17. Explain hypertonic. 18. How is facilitated diffusion different than regular diffusion? 19. Define active transport. 20. What does the sodium / potassium pump do for the cell?

1. What are the structural and functional units of a living organism? 2. What is the role of the cell membrane? 3. What is the cell membrane made of? 4. What does phospholipid bi-layer mean? 5. What are some functions of membrane proteins? 6. What are the two major groups of cell membrane proteins? How are they different? 7. Describe diffusion. 8. What does selectively permeable mean? 9. Does diffusion require energy from the cell? 10. What might restrict the movement of a molecule across a cell membrane? 11. In the video what are channel proteins are compared to? 12. What does gated channel mean? 13. Explain osmosis. 14. Explain isotonic. 15. Explain hypotonic. 16. Explain lysis of a cell. 17. Explain hypertonic. 18. How is facilitated diffusion different than regular diffusion? 19. Define active transport. 20. What does the sodium / potassium pump do for the cell?

1. What is the difference in results between a) conducting a cell permeabilization and b) detergent extraction of a living cell to prepare the cytoskeleton?

2. List the components of homogenization buffer, and what each component does.

3. Why is the nucleus removed by a low speed centrifugation?

4. Why does detergent have to be applied well above the critical micelle concentration when detergent extracting a living cell?

5. Would Tris (also called Trizma) buffer be a good buffer to be placed in "intracellular buffer"? Explain your answer.

6. After detergent extraction, how do you concentrate the soluble fraction? Explain this in detail.

7. What happens when you homogenize a membrane such as the rough endoplasmic reticulum?