For HEPES buffer is pH 4.28, THE pKa is 7.55.The volume of the buffer is 50ml.

a. What is the ratio of A-/ HA in the buffer that is present in the solution?

b. How many micromoles of A- and HA are present in the solution?

c. If you now add 3 ml of 1M NaOH, will you still have a valid buffer? Explain.

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Elin HesselLv2

29 Sep 2019

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1. Given 1 L of 0.05 M sodium bicine (conjugate base of the buffer), how much 0.1 N HCl must be added to make a bicine solution of pH 7.5? What is the molarity of bicine in the final buffer? The pK_a is 8.26.

2. You take 2.38 g of HEPES in the acid form and bring the volume up to 100 mL with water. We will call this solution A. You then add 3 mL of 1 M NaOH to make solution B. Finally you add 7 more mL of 1 M NaOH to make solution C. What are the pH values of the 3 solutions? (The molecular weight of HEPES is 238. 3 g/mol and the pK_a is 7.55; HEPES is a weak acid commonly used for biological buffers.