Question

Dr. Kornberg, a Nobel laureate, recruited you to work in hisresearch lab. He assigned you a project to completely isolate andcharacterize a newly discovered native protein, smoothase. Hebelieves that his protein causes wrinkles to disappear. Preliminarystudies were performed and the results are listed below.
a) The protein bound to a phenyl sepharose column at pH 8.0.
b) Reducing SDS-page analysis resulted in proteins at about 40 and55 kDa.
c) Gel filtration chromatography resulted in a protein at about 135kDa.
d) The protein interacts with a nickel affinity column at pH7.
e) The protein interacts with an anion-exchange column at any pHvalue above 6.0 while it interacts with cation-exchange column atany pH value below 5.0.

A) Describe ALL the characteristics of smoothase that you candetermine from the preliminary data listed above. Be sure toexplain your reasoning.
B) Design a step-by-step purification scheme for smoothase usingthe techniques listed in a-e above and be sure to include any stepsneeded to prepare the samples for the next step and othercomponents (i.e. molecules) needed for efficient elution.