A protein subunit from an enzyme is part of a research study and needs to be characterized. A total of 0.150 g of this subunit was dissolved in enough water to produce 2.00 mL of solution. At 28 C the osmotic pressure produced by the solution was 0.138 atm. What is the molar mass of the protein?

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Answer


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The osmotic pressure of the solution is 0.138 atm.
Temperature of the solution is  or 301 K.
 
The expression for osmotic pressure is:

Here, M is the molarity of the solution.

Rearrange the expression for molarity and substitute the values.


The expression for molarity is:


Rearrange for the expression for moles and calculate the number of moles of enzyme in the solution.


Number of moles of enzyme dissolved in the solution is .
Mass of enzyme dissolved in the solution is 0.150 g.

The formula to calculate the molar mass of the enzyme is:


Substitute the values and calculate the molar mass of the enzyme.

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