(a) Calculate the molar concentration of glucose in the stocksolution.
(b) For each standard solution and the unknown, convert thepercent transmittance to absorbance, using this equation:A=2.000 - log(%T). Enter these absorbences in thetable.
(c) Calculate the molar concentration of glucose complex presentin each solution. Enter the concentrations in the table.
(d) Prepare a Beer's law plot, using the data obtained in (b)and (c).
(e) Using your Beer's law plot, determine the molarconcentration of glucose in the unknown sample
The determination of glucose concentration in blood serum is often based on the formation of a blue-green complex of glucose and o-toluidine in glacial acetic acid. This reaction is shown at the bottom of this page. The analytical wavelength for the complex is 635 nm. A set of standard solutions was prepared by taking a known volume of a stock glucose solution, containing 1.000 g glucose dissolved in 100 mL of distilled water, and diluting to 100 mL with distilled water. These solutions and the blood samples were treated with the o-toluidine reagent, and the percent transmittance of each sample was read against a reference solution. The data obtained are
