The following table shows where different restriction endonucleases(restriction enzymes) cleave DNA. The abbreviation R represents thepurines (adenine and guanine). The pyrimidines (cytosine, thymine,and uracil) are abbreviated as Y. The abbreviation W representsadenine or thymine.
Plasmid DNA and a gene of interest are cut with the enzyme PpuMI.Write a possible seqence of bases for the sticky end of the gene inthe 5' to 3' direction.
The following table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine.
