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11 Nov 2019
s Jst algebra... Below you will derive an equation to express the velocity of an exzyme catalyzed reaction in the presence of a competitive inhibitor Write a kinetic scheme for a s ingle substrate enzyme catalyzed reaction in the presenice of a competitive inhibitor e Just as in the absence of a competitive inhibitor, the rate (velocity) of product formation with a competitive Inhibitor present is () ES Mass balance. In the presence of substrate and competitive inhibitor, the enzyme can be in one of three forms (ill In blanks) I1 Divide. Divide equation (a) by the mass balance equation: Equilibria: ES and El complexes have dissociation constants that can be expressed as (Cill in blanks): c)KI1'I Rearrange equation (c) and (d) to solve for ES and El respectively (ill in blanks) e)IES 1 OIEIlI Substitute. Rewrite equation () with the terns ES and El substituted with the right hand side of (e) and (0) Factor & simplify. Now, on the right hand side of equation (R), I have factored out the [EJ terms, so they cancel, to give (h) [S]/K ninator on t the numerat form of the velocity equation:
s Jst algebra... Below you will derive an equation to express the velocity of an exzyme catalyzed reaction in the presence of a competitive inhibitor Write a kinetic scheme for a s ingle substrate enzyme catalyzed reaction in the presenice of a competitive inhibitor e Just as in the absence of a competitive inhibitor, the rate (velocity) of product formation with a competitive Inhibitor present is () ES Mass balance. In the presence of substrate and competitive inhibitor, the enzyme can be in one of three forms (ill In blanks) I1 Divide. Divide equation (a) by the mass balance equation: Equilibria: ES and El complexes have dissociation constants that can be expressed as (Cill in blanks): c)KI1'I Rearrange equation (c) and (d) to solve for ES and El respectively (ill in blanks) e)IES 1 OIEIlI Substitute. Rewrite equation () with the terns ES and El substituted with the right hand side of (e) and (0) Factor & simplify. Now, on the right hand side of equation (R), I have factored out the [EJ terms, so they cancel, to give (h) [S]/K ninator on t the numerat form of the velocity equation:
Jean KeelingLv2
6 Oct 2019