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11 Nov 2019
BIOL 6733 - Midterm Exam 1. (30p) The structure of the enzyme Tryptophan Synthetase has been studied extensively by a variety of methods. In a series of studies, Yanofsky and co-workers examined the effect on enzyme activity of various amino acid changes in the protein sequence (Federation Proceedings, 22:75 (1963) and Science 146:1593 (1964)). "Wild-type" is the normal strain isolated from the wild. Strain wild type Mutant 1 Mutant 2 Amino Acid Glv Glu Enzymatic Activity Full None Here are two possible explanations for these results i) the glyãglu and gly "> arg changes introduce a charge (+) or Î into a region of the protein that requires an uncharged amino acid like glycine. ii) the gly glu and gly -> arg changes introduce much larger amino acid side-chains into a space in the protein that requires a small amino acid like glycine. Yanofsky & co. collected more mutants and examined their proteins to determine which of the above explanations was more likely to be correct: Strain Amino Acid at position A Enzymatic activity Full Full Full Partial Wild type Mutant 3 Mutant 4 Mutant 5 Ser Ala Val a)Which of their models is supported by this data? Why?
BIOL 6733 - Midterm Exam 1. (30p) The structure of the enzyme Tryptophan Synthetase has been studied extensively by a variety of methods. In a series of studies, Yanofsky and co-workers examined the effect on enzyme activity of various amino acid changes in the protein sequence (Federation Proceedings, 22:75 (1963) and Science 146:1593 (1964)). "Wild-type" is the normal strain isolated from the wild. Strain wild type Mutant 1 Mutant 2 Amino Acid Glv Glu Enzymatic Activity Full None Here are two possible explanations for these results i) the glyãglu and gly "> arg changes introduce a charge (+) or Î into a region of the protein that requires an uncharged amino acid like glycine. ii) the gly glu and gly -> arg changes introduce much larger amino acid side-chains into a space in the protein that requires a small amino acid like glycine. Yanofsky & co. collected more mutants and examined their proteins to determine which of the above explanations was more likely to be correct: Strain Amino Acid at position A Enzymatic activity Full Full Full Partial Wild type Mutant 3 Mutant 4 Mutant 5 Ser Ala Val a)Which of their models is supported by this data? Why?
Trinidad TremblayLv2
20 Mar 2019