Biochem homework!!! Please please please help!!! Just wanna make sure Iâve gotten all of these correct! Thank you!
Measurement of the rate constants for a s ki-2x10 M sec k,,-1x10' sec-1 kcat 50 sec imple enzymatic reaction obeying Michaelis Menten kinetics gave the following results: What is the apparent dissociation constant for the ES complex (K.)? Ignoring keat, what is the true dissociation constant for the ES complex (Ka overestimate or underestimate the lue were similar in magnitude to carbonic anhydrase, (40,000 sec), would the Km actual affinity of the ES complex? If the kinetic measurements were made using 2 nanomoles of enzyme in I mL of buffer and saturating amounts of substrate, what would Vimax equal? (for this question, use kat 50 sec-1) Again using 2 nanomoles of enzyme per l mL of buffer, what concentration of substrate would give 0.7s
