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11 Nov 2019
1. In lecture, we discussed that one utility of FRET was that we can use it to measure distances very precisely between different parts of a molecule or between components of macromolecules. To do so we define the FRET efficiency as kT where kr corresponds to the transfer rate for FRET. This transfer rate is an exit route for an excited 'donor' molecule in addition to the other decay pathway contributions that we previously considered. In other words, the sum of all rate constants related to the decay of an excited state donor molecule concentration is kT kd, where kd is defined exactly as we did in lecture. This FRET efficiency can be equivalently written in terms of a ratio of quantum yields or time con- stants obtained from two different experiments: a first in which the donor quantum yield or time constant is measured when the donor is alone in the sample solution, and a second in which these same quantities are measured for the donor fluorescence while the donor is in the presence of the acceptor. In this question, you will get some practice at converting between rate constants, time constants, and quantum yields by deriving these equivalent expressions for FRET efficiency, PET- (i) Show that the above expression can be rewritten as
1. In lecture, we discussed that one utility of FRET was that we can use it to measure distances very precisely between different parts of a molecule or between components of macromolecules. To do so we define the FRET efficiency as kT where kr corresponds to the transfer rate for FRET. This transfer rate is an exit route for an excited 'donor' molecule in addition to the other decay pathway contributions that we previously considered. In other words, the sum of all rate constants related to the decay of an excited state donor molecule concentration is kT kd, where kd is defined exactly as we did in lecture. This FRET efficiency can be equivalently written in terms of a ratio of quantum yields or time con- stants obtained from two different experiments: a first in which the donor quantum yield or time constant is measured when the donor is alone in the sample solution, and a second in which these same quantities are measured for the donor fluorescence while the donor is in the presence of the acceptor. In this question, you will get some practice at converting between rate constants, time constants, and quantum yields by deriving these equivalent expressions for FRET efficiency, PET- (i) Show that the above expression can be rewritten as
Jarrod RobelLv2
23 Jun 2019