In a cell, if the concentration of Na+in = 10 mM and the concentration of Na+out = 139 mM, what is the value of ÎG for the transport of one mol of Na+ ions into the cell? Assume a temperature of 37 Â°C and a membrane potential of â62 mV (inside of the cell negative relative to outside the cell). Also, F = 23.1 kcal/V mol. Report your answer in terms of kcal/mol to the nearest hundredth of a unit.

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Beverley SmithLv2

22 Oct 2019

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The sodium potassium pump transports 5 ions for every molecule of ATP that is hydrolyzed: 3 Na+ out of the cell and 2 K+ into the cell. The pump maintains internal Na+ at 11 mM and external Na+ at 144 mM; it maintains internal K+ at 139 mM and external K+ at 7 mM. Assuming that the membrane potential is â69 mV (inside negative relative to outside), the temperature is 37 Â°C, and F is 23.1 kcal/VÂ·mol, how much energy does it take to run the pump (i.e., how many kcal of energy are required per 5 mol of ions pumped)? Report your answer to the nearest tenth of a unit.

To what maximum concentration gradient could E. coli accumulate beta-galactosides if its pumping of H+ ions from inside the cell to outside the cell resulted in a ÎpH of 1.5 (interior higher by 1.5 unit) and a membrane potential of â124 mV (interior negative). In other words, imagine that the E. coli are going to use the free energy of the H+ ions coming back into the cell (which they are poised to do) to bring in (or cotransport) beta-galactosides into the cell against their concentration gradient such that the maximum possible gradient allowable is obtained, which is due to the maximum amount of free energy available to do so. Again, the temperature is 37 Â°C, and F = 23.1 kcal/V mol. Report your answer to the nearest ones.

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