Write the reaction of palmitic acid which would occur when the hydrolyzed PC solution is acidified to pH 3 in step #13. Explain why the fatty acid would be preferentially extracted into hexane from the aqueous solution after the pH had been lowered to 3, rather than when the pH was at 8.5 Suppose a TLC separation of a mixture of two substances (A and B) gave the result shown. If the distribution constant (K) is defined as the ratio of moles substance adsorbed to moles substance in solution at equilibrium, which substance, A or B, has the larger K? Explain. A solvent Substance- flow origin