Question

In lab this week, you will be given a sample containing a mixture of two proteins: cytochrome c (pI = 9.5) and ovalbumin (pI = 6.0). Cation exchange chromatography will be used to separate cytochrome c and ovalbumin. Which of the following statements below is CORRECT?

		At a pH of 6.5, cytochrome c will be positively charged and bind to the negatively charged cation exchange resin
		At a pH of 6.5, cytochrome c will be negatively charged and bind to the positively charged cation exchange resin
		At a pH of 6.5, ovalbumin will be positively charged and bind to the negatively charged cation exchange resin
		At a pH of 6.5, ovalbumin will be positively charged and bind to the positively charged cation exchange resin