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11 Nov 2019
Question 1-9 please
your data with a 2 What is the correlation between the color and the concentration of the methanol and Spectrophotometer the acetone? tor may want you to3. Can you suggest a cause for the correlation quantitatively determine the amount betalain present in test tubes 21 26 and 41 recorded above? 46. You can do this with a spectrophotometer set at a wavelength of 520 nm at pH 4ã The collection of individual pigments comprising the betalains has a peak absorbance at 520 nm 4. What do you call test tubes 11 and 31? Why are they included in the experiment? 5. Define the term decant, 6. Why is it necessary to agitate the tubes? and Why is it important to agitate the test tubes 10.2F Disposal of your Reagents 1. Put on your safety goggles. 2. After all students have observed the results, with the same force and for the same amount of time? Why is it necessary to soak the beet plugs at several points in the procedure? How could skipping the soaking steps generate a false positive result or affect the positive results? 7. empty the contents of each test tube into the appropriate waste collection bottle. Do not pour the contents into the sink. 8. In steps 5 and 11 of procedure 10.2B, why is it important for the beet sections to have the same diameters and lengths? 10.2G Graphing your Data Obtain a piece of graph paper and construct a graph showing the effect of the organic 9 From the graph, could you determine the substances on the membranes of the beet cells. Make a progress graph showing approximate damage caused by concentrations other than those that were part of the procedure? Color (y-axis) vs. Percent Concentration (x-axis). If you have obtained absorbance data, plot the absorbance values on the y-axis and percent concentration on the x-axis. Review I: Scientific Investigation for GENERAL REFERENCES information concerning the construction and labeling of your graph. You can use a commercial graphing program if you are Alberts, B., A. Johnson, J. Lewis, M. Raff, K. Roberts, and P. Walter. 2002. Molecular Biology of the procedure to produce the the Cell, 4 Ed. New York, NY: Garland Science, correct graph. If you are not familiar with a commercial graphing program, ask your Becker, W. M., L. J. Kleinsmith, and J. Hardin. 2005. The World of the Cell, 6 Ed. San Francisco, CA: Benjamin/Cummings Publishing Company Horton, R. H., L. A. Moran, R. S. Ochs, J. D. Rawn, and K. G. Scrimgeour. 2002. Principles of Biochemistry, 3d Ed. Upper Saddle River, NJ Prentice-Hall, Inc. the Exercise 10: The Effects of Organic Substances on Cell Membranes
Question 1-9 please
your data with a 2 What is the correlation between the color and the concentration of the methanol and Spectrophotometer the acetone? tor may want you to3. Can you suggest a cause for the correlation quantitatively determine the amount betalain present in test tubes 21 26 and 41 recorded above? 46. You can do this with a spectrophotometer set at a wavelength of 520 nm at pH 4ã The collection of individual pigments comprising the betalains has a peak absorbance at 520 nm 4. What do you call test tubes 11 and 31? Why are they included in the experiment? 5. Define the term decant, 6. Why is it necessary to agitate the tubes? and Why is it important to agitate the test tubes 10.2F Disposal of your Reagents 1. Put on your safety goggles. 2. After all students have observed the results, with the same force and for the same amount of time? Why is it necessary to soak the beet plugs at several points in the procedure? How could skipping the soaking steps generate a false positive result or affect the positive results? 7. empty the contents of each test tube into the appropriate waste collection bottle. Do not pour the contents into the sink. 8. In steps 5 and 11 of procedure 10.2B, why is it important for the beet sections to have the same diameters and lengths? 10.2G Graphing your Data Obtain a piece of graph paper and construct a graph showing the effect of the organic 9 From the graph, could you determine the substances on the membranes of the beet cells. Make a progress graph showing approximate damage caused by concentrations other than those that were part of the procedure? Color (y-axis) vs. Percent Concentration (x-axis). If you have obtained absorbance data, plot the absorbance values on the y-axis and percent concentration on the x-axis. Review I: Scientific Investigation for GENERAL REFERENCES information concerning the construction and labeling of your graph. You can use a commercial graphing program if you are Alberts, B., A. Johnson, J. Lewis, M. Raff, K. Roberts, and P. Walter. 2002. Molecular Biology of the procedure to produce the the Cell, 4 Ed. New York, NY: Garland Science, correct graph. If you are not familiar with a commercial graphing program, ask your Becker, W. M., L. J. Kleinsmith, and J. Hardin. 2005. The World of the Cell, 6 Ed. San Francisco, CA: Benjamin/Cummings Publishing Company Horton, R. H., L. A. Moran, R. S. Ochs, J. D. Rawn, and K. G. Scrimgeour. 2002. Principles of Biochemistry, 3d Ed. Upper Saddle River, NJ Prentice-Hall, Inc. the Exercise 10: The Effects of Organic Substances on Cell Membranes