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11 Nov 2019
I was given a protein sample to determine its sequence. Here are the steps I followed 1. I did an amino acid analysis and got the following Results of the amino acid analysis 5 H2 38 amino acids total D 4 V 1 M 2 Y 2 G 3 2. From the amino acid analysis, I determined that Trypsin and CNBr could be used as two protein cleavage agents to generate smaller fragments of the protein. Think about how did I make that determination? 3. Next, I purified each fragment (for each set of digestions) and sequenced them by Edman's method T-1 GASMALIK T-2EGAAYHDFEPIDPR T-3 DCVHSD T-4 YLIACGPMTK Results of sequencing of peptides obtained from CNBr digestion C-1 EGAAYHDFEPIDPRGASM C-2 TKDCVHSD C-3 ALIKYLIACGPM 4. Help me align the overlapping fragments from the two sets of digestions to know the sequence of the protein!
I was given a protein sample to determine its sequence. Here are the steps I followed 1. I did an amino acid analysis and got the following Results of the amino acid analysis 5 H2 38 amino acids total D 4 V 1 M 2 Y 2 G 3 2. From the amino acid analysis, I determined that Trypsin and CNBr could be used as two protein cleavage agents to generate smaller fragments of the protein. Think about how did I make that determination? 3. Next, I purified each fragment (for each set of digestions) and sequenced them by Edman's method T-1 GASMALIK T-2EGAAYHDFEPIDPR T-3 DCVHSD T-4 YLIACGPMTK Results of sequencing of peptides obtained from CNBr digestion C-1 EGAAYHDFEPIDPRGASM C-2 TKDCVHSD C-3 ALIKYLIACGPM 4. Help me align the overlapping fragments from the two sets of digestions to know the sequence of the protein!
Reid WolffLv2
12 Jun 2019