reduced form of nicotinamide adenine dinucleotide (NADH) is an important The and highly fluorescent coenzyme, with an absorption maximum at 340 nm and a fluorescent emission maximum at 465 nm. Standard solutions of NADH gave 5. the following fluorescence intensities: Conc NADH,umole/l 0.0 0.1 00 0.200 0.300 0.400 0.500 0.600 0.700 0.800 Fl Intensity 0.14 2.24 4.52 6.63 9.01 10.94 13.71 15.49 17.91 An unknown gave a fluorescence of 9.99. Calculate the concentration of the NAD correlation coefficient R. (10 pts) H in the unknown sample. What is the slope of the calibration line and its 6. Three different monochromators were discussed for UV-VIS spectroscopy. Discuss (with diagrams where appropriate) these 3 and what their advantages and disadvantages are. (12 points)