Regarding thin layer chromatography
Name two types of visualization techniques used to see compounds on TLC plates.
What may be the reason for a spot seems to not move from where you spotted the plate?
What would cause two spots to twin?
Regarding thin layer chromatography
Name two types of visualization techniques used to see compounds on TLC plates.
What may be the reason for a spot seems to not move from where you spotted the plate?
What would cause two spots to twin?
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Related questions
If anyone can help me with these two questions, that would be awesome:
1- You perform a TLC and there are large, overlapping, or tailing spots. Why does this happen?
a. | The sample spotted on the plate was too dilute. | |
b. | Too much sample was spotted on the TLC plate. | |
c. | The solvent was spotted above the "starting line". | |
d. | Too much solvent was used to dissolve the compound. |
2- The variable Rf can stand for âRatio to the frontâ. The âfrontâ refers to
a. | the vertical dimension of the TLC plate. | |
b. | the level of the plate that the solvent reaches. | |
c. | the rough side of the silica-loaded TLC plate. | |
d. | the location of the spot that travels the furthest. |