wcsu.blackboard.com 2017) t-rid- https://wcsu.b 32 PART2P Prepare: KFerVal: 0.00200 M stock solution in water. Make 250 mL A typical steady-state quenching experiment involves monitoring the change in emission intensity with quencher concentration. Therefore, from the excitation and emission spectra you obtained for the ruthenium complex, determine the most sensitive conditions for fluorimetric analysis, Le., choose appropriate excitation and emission wavelength settings at which to monitor the fluorescence intensity. Use the excitation wavelength you chose when you took the emission spectrum and monitor the wavelength of the maximum in the emission peak. in this quenching study, the idea is to use a fixed concentration of Ru-bipy against several concentrations of quencher, and record the change in the height of the emission peak as the quencher concentration is varied. The instrument settings are kept fixed for these readings. Each quenching solution is made into a 10-mL volumetric flask, with 2 mL of the Ru- bipy in each fask. The volume is made up to the 10-mL mark either with water (this will be the "unquenched" sample) or with different volumes of quencher solution, in this case KlFe(CNju) plus water. Prepare the follwing in 10-mt volumetrics, using pipettes! solution Q1 02 04 Now set the fluarimeter to the exotation wavelength you found in Part 1 and set the emission wavelength to that of the maximum of the emission peak Place solution Q0 into the Instrument, and adjust the instrument parameters (sensitivity and lamp voltage) to maximize the size of the emission peak. You anly need to scan the emission of the sample just through the emission peak You know at this point where the emission peak is from Part 1 of the ment. With the instrument ogtimized, record the emission of the unquenched sample
