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17 Nov 2019

An enzymatic reaction was analyzed for rate versus substrate concentration. Roughly estimate the Vmax and Km from these measurements. (Hint: Slope = rise/run and remember what Km represents!)

vo(nM/s) [S] (nM)

30 100,000

29.5 10,000

29 1,000

28 200

25 150

20 100

10 50

5 20

a. Vmax = 10 nM/s, Km = 20 nM

b. Vmax = 20 nM/s , Km = 50 nM

c. Vmax = 40 nM/s , Km = 150 nM

d. Vmax = 70 nM/s, Km = 250 nM

ANSWER is (c), how?

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Bunny Greenfelder
Bunny GreenfelderLv2
22 Sep 2019

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