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18 Nov 2019
MDH extracted from pig heart yielded a rate of 1.2 min-1 upon addition of 50 μL extract to a 3 mL assay. show all steps
a) Calculate the amount of MDH activity (Units) per mL of extract
b) The concentration of the extract was 12 mg/mL. Calculate the MDH specific activity (Units/mg) in the crude extract.
c) Pure MDH has a specific activity of 125 μmol min-1 mg-1. How many μg of MDH are in each mL of crude extract?
MDH extracted from pig heart yielded a rate of 1.2 min-1 upon addition of 50 μL extract to a 3 mL assay. show all steps
a) Calculate the amount of MDH activity (Units) per mL of extract
b) The concentration of the extract was 12 mg/mL. Calculate the MDH specific activity (Units/mg) in the crude extract.
c) Pure MDH has a specific activity of 125 μmol min-1 mg-1. How many μg of MDH are in each mL of crude extract?
Deanna HettingerLv2
3 Apr 2019
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