Please help and show full work. Thank you.
consider the unimolecular elementary reversible reaction:
A <--->B
with forward rate constant k1 and backward rate constant k-1.
The reversible folding kinetic equilibrium of a protein assumed to follow the unimolecular reversible
mechanism above (A=Unfolded and B = Folded) . A solution of protein of total concentration C=3.20μM is
investigated. At T=310 K the equilibrium concentration of unfolded proteins is measured to be [U]eq=0.27μM
The temperature of the sample is quickly raised to 330 K and the concentration of unfolded proteins is monitored
as a function of time. After 3.2 ms at the new temperature, the concentration of unfolded proteins increased to
[U]=0.313μM and reached equilibrium at a concentration of [U]eq=0.39μM .
(a) Compute the equilibrium constant for folding at 310 K, and 330 K.
(b) Compute the standard free energy ÎGâ for folding at 310 K, and 330 K and estimate the entropy of folding.
(c) Compute the relaxation time constant at 330 K.
(d) Compute the folding and unfolding rate constants at 330 K.
Please help and show full work. Thank you.
consider the unimolecular elementary reversible reaction:
A <--->B
with forward rate constant k1 and backward rate constant k-1.
The reversible folding kinetic equilibrium of a protein assumed to follow the unimolecular reversible
mechanism above (A=Unfolded and B = Folded) . A solution of protein of total concentration C=3.20μM is
investigated. At T=310 K the equilibrium concentration of unfolded proteins is measured to be [U]eq=0.27μM
The temperature of the sample is quickly raised to 330 K and the concentration of unfolded proteins is monitored
as a function of time. After 3.2 ms at the new temperature, the concentration of unfolded proteins increased to
[U]=0.313μM and reached equilibrium at a concentration of [U]eq=0.39μM .
(a) Compute the equilibrium constant for folding at 310 K, and 330 K.
(b) Compute the standard free energy ÎGâ for folding at 310 K, and 330 K and estimate the entropy of folding.
(c) Compute the relaxation time constant at 330 K.
(d) Compute the folding and unfolding rate constants at 330 K.
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