Put the steps of the mechanism of chymotrypsin catalysis in order from first to last. Note: Your textbook may not break out each of these steps individually, but all steps should be utilized.
first step last step His 57 donates H to N of scissile peptide bond tetrahedral intermediate collapses water donates H to ring N of His 57 His 57 donates H to Ser 195 0 tetrahedral intermediate collapses His 57 ring N deprotonates Ser 195 hydroxyl hydroxide ion attacks carbonyl of remaining N-terminal piece of substrate the C-terminal piece of peptide substrate (with new amino terminus) diffuses away nucleophilic Ser 195 O- attacks carbonyl C of substrate N-terminal piece of peptide substrate (with new carboxyl terminus) diffuses away
