Hypothetical Chromatogranm Solvent Front line 7 6 5 4 3 2 1 Spotting line 0 2) It takes about 0.7ç¡(microliters) of a copper solution containing 0.307 g of copper ion per 100 mL to make a spot approximately 0.397 cm in diameter. Calculate the micrograms of copper ion in the spot. Using the "ruler" in the picture of the chromatogram and measuring with an accuracy of.1 unit, calculate the retardation factor (Re) for compound..C MEASUREMENTS AND CALCULATIONS "units". "units". la) How far did compund C move? lb) At the same time, how far did the solvent move? 1c) What is the Rf for compound C? 2) How manyç¢(micrograms) of copper ion are in the spot? Why do la lh and 2 have units wheras 1c does not?
