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19 Nov 2019
1. Determine the final sequence of a 29aa long peptide from the following data (3 points) a. Treatment of the peptide with Dansyl chloride (used to identify N-terminal amino b. Trypsin digestion, separation of peptides and Edman degradation give sequences for acid) reveals that the amino terminal is Val peptide fragments as follows: Val-Gly-Ala-His-Ala-Gly-Glu-Tyr-Gly-Ala-Glu-Ala-Thr-Glu Ala-Ala-Trp-Gly-Lys Val-Leu-Ser-Pro-Ala-Lys Thr-Asn-Val-Lys Chymotrypsin digestion, separation of peptides, and Edman degradation give the following sequences for fragments: c. Gly-Ala-Glu-Ala-Thr-Glu Gly-Lys-Val-Gly-Ala-His-Ala-Gly-Glu-Tyr Val-Leu-Ser-Pro-Ala-Lys-Thr-Asn-Val-Lys-Ala-Ala-Trp
1. Determine the final sequence of a 29aa long peptide from the following data (3 points) a. Treatment of the peptide with Dansyl chloride (used to identify N-terminal amino b. Trypsin digestion, separation of peptides and Edman degradation give sequences for acid) reveals that the amino terminal is Val peptide fragments as follows: Val-Gly-Ala-His-Ala-Gly-Glu-Tyr-Gly-Ala-Glu-Ala-Thr-Glu Ala-Ala-Trp-Gly-Lys Val-Leu-Ser-Pro-Ala-Lys Thr-Asn-Val-Lys Chymotrypsin digestion, separation of peptides, and Edman degradation give the following sequences for fragments: c. Gly-Ala-Glu-Ala-Thr-Glu Gly-Lys-Val-Gly-Ala-His-Ala-Gly-Glu-Tyr Val-Leu-Ser-Pro-Ala-Lys-Thr-Asn-Val-Lys-Ala-Ala-Trp
Lelia LubowitzLv2
31 Jul 2019