Need help determining total protein in each tissue.
Have already found the initial concentration of each protein from extrapolation and formulas
intial concentration of Liver sample = 21.25 mg/ml
initial concentration od Kidney sample = 5 mg/ml
How do I get total protein in each tissue in mg / g?
See below image-- Biochemistry
NOTE: To determine the initial concentration of the protein in an unknown sample, one must extrapolate values from the standard curve and correct for dilution of the sample Questions: You have been called upon to assist Dr. Brosnan with an on-going research project. Dr Brosnan believes that her new protein may exist not only in the liver but also in the kidney You have been asked to determine the TOTAL PROTEIN per gram of tissue using the "Biuret Method" for protein determination. On the day of your experiment, a rat is killed and the liver and kidneys are removed. Each tissue is weighed and placed in homogenization medium at a ratio of approximately 9 ml per gram of liver tissue and 3 ml per gram of kidney tissue. The tissues are homogenized in a potter-elvejham with eight passes of the teflon pestle. The actual procedure is listed in the table below. MAKE SURE TO USE THIS DATA FOR YOUR CALCULATIONS Homogenization Procedure: Wet weight of tissue (g) Homogenization medium added (ml) Tissue Sample Liver Kidnev 9.25 2.45 8.75 In the afternoon, you perform the Biuret assay to determine the total protein in each gram of wet tissue. Your BSA standard is 15 mg protein /ml. Co Table 2 and calculate the total protein in each tissue Upon completion of your assigned Work you are asked to perform a second tasK. DI. Brosnan wishes to know if the protein is definitely found in both tissues. From your previous lab. what procedure would you suggest that she use to solve this issue? Please state the exact procedure you would use and support your choice standard curve using the data in Table 2 Water (ml) Biuret Absorbance Protein/Unknown | Volume used | 5% DOC Tissue None Standard 1 Standard 2 Standard 3 Liver Homogenate Kidnev Homogenate (ml) In 0.2 0.2 0.2 0.2 0.2 0.8 0.7 0.6 0.4 0.6 Reagent (ml)|a 560 nm 4.0 4.0 4.0 4.0 4.0 0.09 0.21 0.33 0.57 0.42 0.2 0.2 0.2 0.4 4.0 0.28 uestions Cont'd
