A protein of interest needs to be separated from a mixture of proteins for amino acid sequencing. It is known that the protein of interest is highly enriched in lysine residues compared to the other proteins in the mixture. Which of the following techniques will be most effective in isolating this protein?
options:
a) gel-filtration chromatography
b) SDS-polyacrylamide gel electrophoresis
c) mass spectrometry
d) cation exchange chromatography
e) anion exchange chromatography
2. Consider a mixture of three peptides. The three peptides have the following primary structure:
A) asp-asp-val
B) glu-asp-glu
C) val-val-lys
Determine the net charge of each peptide at pH 7. From this, in what order will these peptides elute from an anion exchange column at pH 7?
options:
a) A will elute first, followed by B then C.
b) B will elute first, followed by C then A.
c) C will elute first, followed by A then B.
d) B will elute first, followed by A then C.
e) Not enough information is provided to determine the elution order.
A protein of interest needs to be separated from a mixture of proteins for amino acid sequencing. It is known that the protein of interest is highly enriched in lysine residues compared to the other proteins in the mixture. Which of the following techniques will be most effective in isolating this protein?
options:
| a) | gel-filtration chromatography | ||||||||||
| b) | SDS-polyacrylamide gel electrophoresis | ||||||||||
| c) | mass spectrometry | ||||||||||
| d) | cation exchange chromatography | ||||||||||
| e) | anion exchange chromatography 2. Consider a mixture of three peptides. The three peptides have the following primary structure: A) asp-asp-val B) glu-asp-glu C) val-val-lys Determine the net charge of each peptide at pH 7. From this, in what order will these peptides elute from an anion exchange column at pH 7? options:
|
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Related questions
1. Consider a mixture of three peptides. The three peptides have the following primary structure: A) asp-asp-val B) glu-asp-glu C) val-val-lys
Determine the net charge of each peptide at pH 7. From this, in what order will these peptides elute from an anion exchange column at pH 7?
options:
a) A will elute first, followed by B then C.
b) B will elute first, followed by C then A.
c)C will elute first, followed by A then B.
d) B will elute first, followed by A then C.
e)Not enough information is provided to determine the elution order.
2. Which of the following statements regarding chirality and amino acids is true?
options:
| a) | Alanine has 2 chiral carbons |
| b) | Leucine has three chiral carbons. |
| c) | Isoleucine has two chiral carbons. |
| d) | Aspartic acid has three chiral carbons |
3. The following are statements regarding structural features of the single subunit peptide in the diagram provided. Which statement is correct?
options:
| a) | Interaction "c" represents quaternary structure. |
| b) | Interaction "e" is more likely to be sensitive to changes in pH than interaction "d". |
| c) | Interaction "b" is the result of the reduction of two methionine amino acid residues. |
| d) | The R groups in the region labeled "a" point to the interior of the alpha helix. |
| e) | Interactions b, c, d and e are all noncovalent. |
