how does an oxygen equilibrium curve work? in the context of this clip from a paper
Oxygen equilibrium curves (OECs). All OECs were deter- mined on a HEMOX analyser (TCS, Southampton, PA, USA). Approximately 15±20 ml of fresh heparinized blood was studied at 37VC in N-tris(hydroxymethyl)methyl-2-amino- ethanesulphonic acid (TES) buffer (30 mmol/l, pH 7 Ì6) containing 134 mmol/l NaCl, 5 mmol/l KCl, 8 mmol/l glucose, 0 Ì1% BSA, and 0 Ì01% antifoam (SAG-10, Union Carbide, Danbury, CT, USA) (Asakura, 1979). Reported P50 values are averages of determinations from two to five independent animals. Purified Hbs were studied at 25VC in potassium phosphate buffer (100 mmol/l, pH 7 Ì4) supple- mented with 0 Ì01% antifoam and 0 Ì04% hexamethylpho- sphoramide (HMPA) (Fisher, Fair Lawn, NJ, USA) as the stabilizing agent (Asakura et al, 1978). P50 values represent the average of three or more determinations of independent aliquots taken on at least two separate days. Bohr-effect studies were carried out at 25VC in 100 mmol/l potassium phosphate buffer adjusted to defined pHs. The allosteric effects of supplementary 2,3-BPG (Sigma, St. Louis, MO, USA) were determined at 37VC in Bis-Tris buffer (50 mmol/l, pH 7 Ì4) containing HMPA, antifoam, and 100 mmol/l NaCl (Hofmann et al, 1995).
how does an oxygen equilibrium curve work? in the context of this clip from a paper
Oxygen equilibrium curves (OECs). All OECs were deter- mined on a HEMOX analyser (TCS, Southampton, PA, USA). Approximately 15±20 ml of fresh heparinized blood was studied at 37VC in N-tris(hydroxymethyl)methyl-2-amino- ethanesulphonic acid (TES) buffer (30 mmol/l, pH 7 Ì6) containing 134 mmol/l NaCl, 5 mmol/l KCl, 8 mmol/l glucose, 0 Ì1% BSA, and 0 Ì01% antifoam (SAG-10, Union Carbide, Danbury, CT, USA) (Asakura, 1979). Reported P50 values are averages of determinations from two to five independent animals. Purified Hbs were studied at 25VC in potassium phosphate buffer (100 mmol/l, pH 7 Ì4) supple- mented with 0 Ì01% antifoam and 0 Ì04% hexamethylpho- sphoramide (HMPA) (Fisher, Fair Lawn, NJ, USA) as the stabilizing agent (Asakura et al, 1978). P50 values represent the average of three or more determinations of independent aliquots taken on at least two separate days. Bohr-effect studies were carried out at 25VC in 100 mmol/l potassium phosphate buffer adjusted to defined pHs. The allosteric effects of supplementary 2,3-BPG (Sigma, St. Louis, MO, USA) were determined at 37VC in Bis-Tris buffer (50 mmol/l, pH 7 Ì4) containing HMPA, antifoam, and 100 mmol/l NaCl (Hofmann et al, 1995).
Related textbook solutions
Basic Chemistry
Principles of Chemistry Molecular Approach
Principles of Chemistry Molecular Approach
Chemistry: Structure and Properties
Chemistry: A Molecular Approach
Chemistry: A Molecular Approach
Principles of Chemistry: A Molecular Approach
