How many grams of sodium biphosphate (173 g/mol) and mL of HCl or NaOH, and mL of water are needed to make 500 mL of 0.15 M phosphate buffer (pH 11.90) And what would be the pH of this solution if it were diluted to 2 mL with pure water? pKa(1) = 2.15 pKa2 = 6.86 pKa(3) = 12.38

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Deanna HettingerLv2

13 Dec 2019

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Sodium Phosphate buffer has 3 ionizable groups, with pKa's of 2.15, 7.20 and 12.45. A 1.00 M solution of this buffer (200 mL) is found to have a pH of 7.50 A biochemist adds 30.0 mL of 1.00 M HCL to this buffer.

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