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12 Dec 2019
Determine the quantity of each chemical that you are going to use for preparing the buffer. Calculate the amount you will need to weigh out.
Stock Solutions to Prepare:
a.) 0.5 L of 0.2 M monobasic sodium phosphate (A)
b.) 0.1 L of 0.2 M dibasic sodium phosphate (B) (sometimes precipitation is observed over time so this should be made freshly)
c.) 100 mL of 1 M Tris base pH 8
d.) 250 mL of 4 M NaCl
e.) 20 mL of 4 M imidazole
f.) 10 mL of 0.5 M MgCl2
Estimate Buffer pH Adjustment
To calculate the amount of acid needed to adjust the pH of the Tris base (pKa = 8.3) to pH 8 use the Henderson-Hasselbalch Equation.
Determine the quantity of each chemical that you are going to use for preparing the buffer. Calculate the amount you will need to weigh out.
Stock Solutions to Prepare:
a.) 0.5 L of 0.2 M monobasic sodium phosphate (A)
b.) 0.1 L of 0.2 M dibasic sodium phosphate (B) (sometimes precipitation is observed over time so this should be made freshly)
c.) 100 mL of 1 M Tris base pH 8
d.) 250 mL of 4 M NaCl
e.) 20 mL of 4 M imidazole
f.) 10 mL of 0.5 M MgCl2
Estimate Buffer pH Adjustment
To calculate the amount of acid needed to adjust the pH of the Tris base (pKa = 8.3) to pH 8 use the Henderson-Hasselbalch Equation.
Trinidad TremblayLv2
13 Dec 2019