Question

You need to make 50 mL of a 25 mM Tris buffer that has a pH of 8.40. Show calculations for and describe how you would make it if you had access to: •A) Solid Tris acid (Tris HCl) and solid Tris base •B) Solid Tris acid and 1.0 M NaOH •C) Solid Tris base and 1.0 M HCl