Question

Buffer Calculations Practice (how much to mix?)

PART A:

Make a 300 ml solution of 100mM Tris base in H₂O. CALCUALTE

Use the henderson hasselbach to make a 50 ml 50mM Tris solution at pH 8.0 by mixing Tris HCl and Tris base powders ( use formula weights of Tris-HCl and Tris base). CALCULATE How many grams of Tris HCl and Tris base did you add to make your solution?

PART B: HOW DO YOU MAKE THE FOLLOWING BUFFER; CALCULATE

Buffer: 10X TAE

0.4M Tris base

0.01M disodium EDTA

0.2M acetic acid

Make 500ml of this solution from chemical stocks. The pH of this solution should be between 8.2 and 8.3.

THEN Make a 100 ml 1X dilution of TAE from this 10X stock. CALCULATE