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13 Dec 2019
In the study of biochemical processes, a commonbuffering agent is the weak basetrishydroxymethylaminomethane,(HOCH2)3CNH2,often abbreviated as Tris. At 25 ?C, Tris has a pKb of5.91.
The hydrochloride of Tris is (HOCH2)3CNH3Cl, which can be abbreviated as TrisHCl.
What volume of 10.0 M NaOH is needed to prepare abuffer with a pHof 7.79 using 31.52 gof TrisHCl?
ANSWER: 6.7 mL
Question on this part:
The buffer from Part A is diluted to1.00 L. To half of it (500. mL), you add0.0300molof hydrogen ions without changing the volume.What is the pHof the final solution?
In the study of biochemical processes, a commonbuffering agent is the weak basetrishydroxymethylaminomethane,(HOCH2)3CNH2,often abbreviated as Tris. At 25 ?C, Tris has a pKb of5.91.
The hydrochloride of Tris is (HOCH2)3CNH3Cl, which can be abbreviated as TrisHCl.
What volume of 10.0 M NaOH is needed to prepare abuffer with a pHof 7.79 using 31.52 gof TrisHCl?
ANSWER: 6.7 mL
Question on this part:
The buffer from Part A is diluted to1.00 L. To half of it (500. mL), you add0.0300molof hydrogen ions without changing the volume.What is the pHof the final solution?
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