Labeled pyruvate containing 14C ( 200 mCi/gm at ) C-3 ( the methyl carbon), 13 C( 90% enriched) at C-2 and 18 O ( 80%enriched) at C-2m was added to a pigeon breast muscle preparation, in order to investigate the citric acid cycle.

What will the labeling patern be ( both postion and extent of labeling ) in the isolated a- ketogluterate and in oxaloacetate ( Assume label enters onlin in the first turn ( ie pulse of labeled pyruvate is followed by a chase of unlabeled pyruvate.))

1st cycle 2nd cycle 3rd cycle

-- A ketoglutarate

18O

14 C

13 C

--- Oxaloacetate

18O

14 C

13 C

Question

Labeled pyruvate containing 14C ( 200 mCi/gm at ) C-3 ( the methyl carbon), 13 C( 90% enriched) at C-2 and 18 O ( 80%enriched) at C-2m was added to a pigeon breast muscle preparation, in order to investigate the citric acid cycle.

What will the labeling patern be ( both postion and extent of labeling ) in the isolated a- ketogluterate and in oxaloacetate ( Assume label enters onlin in the first turn ( ie pulse of labeled pyruvate is followed by a chase of unlabeled pyruvate.))

	1st cycle	2nd cycle	3rd cycle
-- A ketoglutarate 18O
14 C
13 C
--- Oxaloacetate 18O
14 C 13 C

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