Question

An investigator starts an enzymatic assay with [S] >> [Et]. (The enzyme is a single-substrate enzyme that produces a single product and is known to follow Michaelis-Menten kinetics.) The investigator monitors the reaction until equilibrium is reached, and then she adds more enzyme to the reaction mixture. The amount added brings the total amount of enzyme to double the original amount. Assuming the volume change upon addition of extra enzyme is negligible, which of the following statements is an accurate assessment of what the investigator could reasonably expect to happen? Select all that apply.

1.	Both the forward and reverse reactions would continue to proceed, but the reverse reaction would proceed faster than the forward reaction.

2.	A new position of equilibrium would be established.

3.	There would be a new burst of net substrate formation.

4.	There would be a new burst of net product formation.

5.	Both the forward and reverse reactions would continue to proceed, but the forward reaction would proceed faster than the reverse reaction.

6.	None of the other choices is correct.